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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has long been observed in many tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was uncovered to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a crucial purpose in advertising mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, happen to be described. Two supplemental splice variants had been claimed but detailed nucleotide sequence facts isn't nevertheless out there.

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Blocking peptides are peptides that bind exclusively towards the concentrate on antibody and block antibody binding. These peptide usually consists of the epitope recognized via the antibody.

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This tutorial outlines additional attainable optimization of cellular and immunoassay parameters to guarantee the very best results are...

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This manual outlines further achievable optimization of mobile and immunoassay parameters to be sure the best possible effects are...

Antibodies sure to the blocking peptide no longer bind on the epitope over the goal protein. By evaluating the staining from your blocked antibody vs . the antibody on your own, one can see which staining is precise.

Concentrate on Information and facts Cyclin E belongs to the extremely conserved cyclin spouse and children, whose members are characterized by a remarkable periodicity in protein abundance throughout website the cell cycle. Cyclins perform as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and functions for a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates at the G1-S stage boundary and is degraded as cells progress through S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...

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